Korormicin, an Antibiotic Specific for Gram-negative Marine Bacteria, Strongly Inhibits the Respiratory Chain-linked Na + -Translocating NADH: Quinone Reductase from the Marine Vibrio alginolyticus kazuhiro yoshikawa*, yuji nakayamaf,
نویسندگان
چکیده
Marine bacteria require a significant amount of NaCl for optimal growth and have quite different physiological character from terrestrial counterparts. For example, marine bacteria possess a unique type ofNADH:quinone reductase, a membrane-boundenzymewhich takes part in the respiratory chain1}. This enzyme requires Na+ for its activity and with its Na+-pumping activity generates sodium-motive force which enables these bacteria to live easily in the marine environment. This energy-acquisition system was found only in Gram-negative marine and moderately halophilic bacteria but not in halo-tolerant bacteria or archaea which also live in the environment with high salinity2). In this regard it is probable that marine bacteria produce unique metabolites by their
منابع مشابه
The mode of action of nanaomycins D and A on a gram-negative marine bacterium Vibrio alginolyticus.
Nanaomycin (NNM) D had a higher growth inhibitory activity than NNM-A against a Gram-negative marine bacterium, Vibrio alginolyticus. These quinone antibiotics were reduced by the respiratory chain-linked flavin dehydrogenase of the organism and the reduced forms of NNMs were quickly autoxidized by molecular oxygen to produce superoxide radicals (O2-). NNM-D was more effective than NNM-A both i...
متن کاملIsolation and molecular identification of Vibrio alginolyticus from cultured marine fish in farms located south provinces of Iran
Bacterial diseases are a major challenge and warning to the development of aquaculture industry. Vibriosis is caused by bacteria of the genus Vibrio, especially Vibrio alginolyticus, in warm and temperate regions, which cause severe damages to the marine fish production economy every year. The aim of the present study was to isolate and identify this bacterium from farmed marine fish with bacte...
متن کاملType VI Secretion System Toxins Horizontally Shared between Marine Bacteria
The type VI secretion system (T6SS) is a widespread protein secretion apparatus used by Gram-negative bacteria to deliver toxic effector proteins into adjacent bacterial or host cells. Here, we uncovered a role in interbacterial competition for the two T6SSs encoded by the marine pathogen Vibrio alginolyticus. Using comparative proteomics and genetics, we identified their effector repertoires. ...
متن کاملComplete Genome Sequence of Vibrio alginolyticus ATCC 17749T
Vibrio alginolyticus is a Gram-negative halophilic bacterium and has been recognized as an opportunistic pathogen in both humans and marine animals. It is the causative agent of food-borne diseases, such as gastroenteritis, and it invades through wounds in predisposed individuals. In this study, we present the completed genome of V. alginolyticus ATCC 17749(T) through high-throughput sequencing.
متن کاملNMR reveals double occupancy of quinone-type ligands in the catalytic quinone binding site of the Na+-translocating NADH:Quinone oxidoreductase from Vibrio cholerae.
The sodium ion-translocating NADH:quinone oxidoreductase (Na(+)-NQR) from the pathogen Vibrio cholerae exploits the free energy liberated during oxidation of NADH with ubiquinone to pump sodium ions across the cytoplasmic membrane. The Na(+)-NQR consists of four membrane-bound subunits NqrBCDE and the peripheral NqrF and NqrA subunits. NqrA binds ubiquinone-8 as well as quinones with shorter pr...
متن کامل